Topical administration of deep sea shark liver oil (DESSLO™) inhibit Nuclear Factor-kappa Beta (NF-κB) expression in Wistar rats (Rattus Norvegicus) skin exposed to ultraviolet-B

  • Devita Horax Master Program in Biomedical Sciences, Anti-Aging Medicine Concentration; Faculty of Medicine, Universitas Udayana, Denpasar, Bali
  • A. A. G. P. Wiraguna Department of Dermatology and Venerology, Faculty of Medicine, Universitas Udayana, Denpasar, Bali
  • Gde Ngurah Indraguna Pinatih Department of Public Health, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Abstract

Introduction: Repeated exposure of the skin to UV-B rays induce reactive oxygen species (ROS) formation that activates Nuclear Factor-kappa Beta (NF-κB) and stimulates pro- inflammatory cytokines expression which leads to premature aging of skin. Deep-sea shark liver oil contains ±80% squalene which could help neutralized ROS. Therefore, the purpose of this study was to assess the effect of topical deep sea shark liver oil toward NF-κB expression in UV-B-exposed on wistar rat (Rattus norvegicus) skin.


Methods: A true experimental posttest-only control study was conducted using 36 Wistar rats which divided into 2 groups (n= 18). Control group was exposed to UV-B and treated with topical placebo basis of glycerin while the treatment group was treated with topical deep sea shark liver oil. A total of 840 mJ/cm2 UV-B exposure was given for 4 weeks. NF-κB expression was examined by immunohistochemistry.


Results: The  mean  NF-κB  expression was significantly higher in control  group  at 30.8±3.81  while  the treatment  group  treated  with  topical  deep  sea  shark  liver  oil has average expression at 10.4±1.98. The independent t-test showed a t-value of 20.090, and a p-value of 0.033. 


Conclusion: It can be concluded that topical deep sea shark  liver  oil  inhibits  the expression of NF-κB expression in UV-B-exposed Wistar rat (Rattus norvegicus) skin.

Author Biographies

Devita Horax, Master Program in Biomedical Sciences, Anti-Aging Medicine Concentration; Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Master Program in Biomedical Sciences, Anti-Aging Medicine Concentration; Faculty of Medicine, Universitas Udayana, Denpasar, Bali

A. A. G. P. Wiraguna, Department of Dermatology and Venerology, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Department of Dermatology and Venerology, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Gde Ngurah Indraguna Pinatih, Department of Public Health, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Department of Public Health, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Published
2020-06-01
How to Cite
HORAX, Devita; WIRAGUNA, A. A. G. P.; PINATIH, Gde Ngurah Indraguna. Topical administration of deep sea shark liver oil (DESSLO™) inhibit Nuclear Factor-kappa Beta (NF-κB) expression in Wistar rats (Rattus Norvegicus) skin exposed to ultraviolet-B. IJAAM (Indonesian Journal of Anti-Aging Medicine), [S.l.], v. 4, n. 1, p. 5-7, june 2020. ISSN 2615-5079. Available at: <https://ijaam-unud.org/ojs/index.php/ijaam/article/view/40>. Date accessed: 02 dec. 2020. doi: https://doi.org/10.36675/ijaam.v4i1.40.