Black rice bran (oryza sativa l. indica) extract cream prevented the increase of dermal matrix metalloproteinase-1 and dermal collagen reduction of male Wistar rats (rattus norvegicus) exposed to ultraviolet-B rays

  • Astried Haryanto Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Udayana University Denpasar
  • Wimpie Pangkahila Department of Andrology and Sexology, Faculty of Medicine, Udayana University
  • Anak Agung Gde Putra Wiraguna Department of Dermatology Faculty of Medicine, Udayana University

Abstract

Introduction: The radiation of UV B rays exposure is capable of inducting free radicals to the skin, damaging the dermal collagen and stimulating skin aging. Photoaging prevention may be conducted by utilizing antioxidant and one of the good sources of antioxidant is black rice bran. Therefore the purpose of this research was to assess the effects of topical 35% black rice bran (Oryza sativa L.indica) extract toward dermal Matrix Metalloproteinase-1 (MMP-1) and dermal collagen in male Wistar rats (Rattus norvegicus).


Method: A post-test only control group design was conducted using 36 male Wistar rats divided into 2 groups (treatment and control). Both groups were exposed to UV-B rays 3 times a week for 4 weeks but the treatment group received 35% black rice bran extract cream twice a day. The control group was only treated by base cream. Histopathological examination was used to assess MMP-1 expression while Picro Sirius Red staining was used to assess dermal collagen on the rat’s skin.


Result: Comparative statistical analysis results for both groups revealed that MMP-1 expression in treatment group was significantly lower than control group (30.94±4.22% vs. 9.57±2.76%; p<0.001). Meanwhile, collagen density was significantly higher in treatment group (83.59±2.84% vs. 58.40±2.69%).


Conclusion: Topical application of 35% black rice bran extract cream prevented the increase of MMP-1 expression and dermal collagen reduction. Further study is needed to validate these findings.

Author Biographies

Astried Haryanto, Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Udayana University Denpasar

Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Udayana University Denpasar

Wimpie Pangkahila, Department of Andrology and Sexology, Faculty of Medicine, Udayana University

Department of Andrology and Sexology, Faculty of Medicine, Udayana University

Anak Agung Gde Putra Wiraguna, Department of Dermatology Faculty of Medicine, Udayana University

Department of Dermatology Faculty of Medicine, Udayana University

Published
2020-06-01
How to Cite
HARYANTO, Astried; PANGKAHILA, Wimpie; WIRAGUNA, Anak Agung Gde Putra. Black rice bran (oryza sativa l. indica) extract cream prevented the increase of dermal matrix metalloproteinase-1 and dermal collagen reduction of male Wistar rats (rattus norvegicus) exposed to ultraviolet-B rays. IJAAM (Indonesian Journal of Anti-Aging Medicine), [S.l.], v. 4, n. 1, p. 16-19, june 2020. ISSN 2615-5079. Available at: <https://ijaam-unud.org/ojs/index.php/ijaam/article/view/48>. Date accessed: 29 oct. 2020. doi: https://doi.org/10.36675/ijaam.v4i1.48.