The application of black garlic (Allium sativum linn) extract cream in preventing the increase level of MMP-1 expression and the decrease of dermal collagen count in the skin of male wistar rats (Rattus norvegicus) exposed to UV-B Rays

  • Balqis Wasliati Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Universitas Udayana Denpasar
  • Wimpie Pangkahila Department of Andrology and Sexology, Faculty of Medicine, Universitas Udayana, Denpasar, Bali
  • Anak Agung Gede Putra Wiraguna Department of Dermatology and Venerology, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Abstract

Introduction: UV-B exposure triggers the activation of Protein-1 (AP-1) which disrupts the integrity of the extracellular matrix by inducing MMP-1. Black garlic causes an increase of antioxidant content namely phenolic, flavonoid (quercetin), and DPPH (IC-50). This study aimed to determine the application of black garlic (Allium sativum linn) extract cream in preventing the increased level of MMP-1 expression and the decrease of dermal collagen count in the skin of male Wistar rats exposed to UV-B rays.


Methods: This study employed an experimental Post Test Only Control Group Design using 32 male Wistar rats. The rats that were applied with base cream material and exposed to UV-B rays were called the control group. The rats that were applied with 15% black garlic extract cream and exposed to UV-B rays were called the treatment group. The expression of MMP-1 was examined by immunohistochemistry and the dermal collagen count of the skin using Pico-Sirius-Red staining.


Results: The mean expression of MMP-1 of the control group was 28.58% and the treatment group was 8.60% (p <0.001). The mean count of collagen in the control group was 57.53% and the treatment group was 83.80% (p <0.001). There was a significant difference in the MMP-1 expression and the count of collagen between the treatment of the control group and the treatment group.


Conclusion: Administration of the cream of 15% black garlic extract was proven to prevent an increase MMP-1 expression and decrease dermal collagen count in the skin of male Wistar rats exposed to UV-B rays.



Author Biographies

Balqis Wasliati, Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Universitas Udayana Denpasar

Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Universitas Udayana Denpasar

Wimpie Pangkahila, Department of Andrology and Sexology, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Department of Andrology and Sexology, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Anak Agung Gede Putra Wiraguna, Department of Dermatology and Venerology, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Department of Dermatology and Venerology, Faculty of Medicine, Universitas Udayana, Denpasar, Bali

Published
2021-12-01
How to Cite
WASLIATI, Balqis; PANGKAHILA, Wimpie; WIRAGUNA, Anak Agung Gede Putra. The application of black garlic (Allium sativum linn) extract cream in preventing the increase level of MMP-1 expression and the decrease of dermal collagen count in the skin of male wistar rats (Rattus norvegicus) exposed to UV-B Rays. IJAAM (Indonesian Journal of Anti-Aging Medicine), [S.l.], v. 5, n. 2, p. 88-92, dec. 2021. ISSN 2615-5079. Available at: <https://ijaam-unud.org/ojs/index.php/ijaam/article/view/81>. Date accessed: 17 may 2022. doi: https://doi.org/10.36675/ijaam.v5i2.81.