The application of kenikir (Cosmos caudatus) extract cream to inhibit the increase of melanin counts in the skin of guinea pigs (Cavia porcellus) exposed to ultraviolet B

  • Olivia Olivia Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Universitas Udayana Denpasar`
  • I Gusti Made Aman Department of Pharmacology Medical Faculty Universitas Udayana Denpasar
  • AAGP Wiraguna Department of Dermatology and Venerology, Faculty of Medicine, Universitas Udayana

Abstract

Introduction: Hyperpigmentation is caused by the increase of melanin in the skin layer, especially the epidermal layer. Kenikir  (Cosmos Caudatus) is a plant that can be used as an antioxidant and anti-melanogenic. The aim of this research was to prove the effectiveness of the cream with 4% kenikir  (Cosmos Caudatus) extract to inhibit the increase of melanin in the skin of guinea pig (Cavia porcellus) exposed to ultraviolet B.


Methods: The kenikir  (Cosmos Caudatus) that have been extracted in Food Laboratory of Agriculture Faculty in Universitas Udayana became the main requirement in this research. This research employed an experimental design with post-test only control group method. To conduct this study, 36 male guinea pigs (Cavia porcellus), local strain, aged 3 months with 300-350mg body weight divided into 2 groups, each of which was 18. The duration of irradiation was 3 times a week with a total irradiation dose of 65 mJ/cm2. After 2 weeks of research, then the back skin tissue of guinea pigs biopsied as the histological preparations and then the Masson-Fontana staining was performed. The results were analyzed by using descriptive analysis, the test of normality and homogeneity and also independent t-test.


Results: The control group (P0) (20,57±2,90%) had a bigger average melanin count than the treatment group (P1) (1,41±0,437%) significant statistically (p<0.001).


Conclusion: this research is the cream with 4% of kenikir  (Cosmos Caudatus) extract can inhibit the increase of melanin counts in the skin of a male guinea pig (Cavia porcellus) exposed to Ultraviolet B.


 

Author Biographies

Olivia Olivia, Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Universitas Udayana Denpasar`

Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Universitas Udayana Denpasar

I Gusti Made Aman, Department of Pharmacology Medical Faculty Universitas Udayana Denpasar

Department of Pharmacology Medical Faculty Universitas Udayana Denpasar

AAGP Wiraguna, Department of Dermatology and Venerology, Faculty of Medicine, Universitas Udayana

Department of Dermatology and Venerology, Faculty of Medicine, Universitas Udayana

Published
2021-12-01
How to Cite
OLIVIA, Olivia; AMAN, I Gusti Made; WIRAGUNA, AAGP. The application of kenikir (Cosmos caudatus) extract cream to inhibit the increase of melanin counts in the skin of guinea pigs (Cavia porcellus) exposed to ultraviolet B. IJAAM (Indonesian Journal of Anti-Aging Medicine), [S.l.], v. 5, n. 2, p. 72-74, dec. 2021. ISSN 2615-5079. Available at: <https://ijaam-unud.org/ojs/index.php/ijaam/article/view/86>. Date accessed: 17 may 2022. doi: https://doi.org/10.36675/ijaam.v5i2.86.